Extracellular Polysaccharide of Erwinia amylovora : a Correlation with Virulence

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Extracellular Polysaccharide of Erwinia amylovora: a Correlation with Virulence.

The extracellular polysaccharides produced as slime or capsule layers by bacterial pathogens of animals and plants have been often implicated as factors essential to pathogenesis. In the present study, virulence of the plant pathogen Erwinia amylovora was correlated with the ability to produce extracellular polysaccharide (EPS). EPS production by a series of field isolates and bacterio-phage-re...

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Fine Structure of Extracellular Polysaccharide of Erwinia amylovora.

Virulent E(9) and avirulent E(8) strains of Erwinia amylovora were shown by means of light, transmission, and scanning microscopy to be, respectively, encapsulated and unencapsulated. Difficulty was encountered in stabilizing the fibrillar-appearing capsular extracellular polysaccharide. We suggest that the ephemeral nature of extracellular polysaccharide is due to the collapse of its extended ...

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Virulence Factors of Erwinia amylovora: A Review

Erwinia amylovora, a Gram negative bacteria of the Enterobacteriaceae family, is the causal agent of fire blight, a devastating plant disease affecting a wide range of host species within Rosaceae and a major global threat to commercial apple and pear production. Among the limited number of control options currently available, prophylactic application of antibiotics during the bloom period appe...

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Partial Purification and Characterization of a Polysaccharide Depolymerase Associated with Phage-Infected Erwinia amylovora.

Erwinia amylovora infected with bacteriophage ERA103 produced an enzyme which degraded the extracellular polysaccharide of noninfected cells. The depolymerase enzyme was purified 15-fold by a procedure which included ammonium sulfate precipitation, ultracentrifugation, CM-Sephadex batchwise separation, Sephadex G-50 column chromatography, and Sephacryl S-200 column chromatography. The enzyme ha...

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Bacteriophages of Erwinia amylovora.

Fifty bacteriophage isolates of Erwinia amylovora, the causal agent of fire blight, were collected from sites in and around the Niagara region of southern Ontario and the Royal Botanical Gardens, Hamilton, Ontario. Forty-two phages survived the isolation, purification, and storage processes. The majority of the phages in the collection were isolated from the soil surrounding trees exhibiting fi...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1979

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.38.4.659-666.1979